Roles of dendritic cells treated with 17?-estradiol in immune tolerance induction in skin allograft
- VernacularTitle:雌激素处理DCs在诱导小鼠同种皮肤移植免疫耐受中的作用
- Author:
Yuan SHEN
;
Yaoying ZENG
;
Jingxian ZHAO
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Estradiol;
Immune tolerance;
Skin transplantation;
Mice
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the roles of bone marrow-derived dendritic cells from donor mouse treated with 17?-estradiol(E2)in immune tolerance induction in skin allograft.METHODS:Bone marrow-derived dendritic cells from C57 mouse as donor were cultured respectively treated with E2(E2 group).BALB/c mouse as recipient received respectively one injection of dendritic cells of E2 group,mature dendritic cell group and immature dendritic cell group intravenously.Skin transplantation was performed in the absence of immunosupression after 7 d.Mice that received PBS were served as control.The time of skin survival was observed after transplantation.Flow cytometry was used to analyze the percentage of CD4+CD25+ T cells in peripheral blood respectively before and after transplantation.RESULTS:Compared with immature dendritic cells and control group,the time of skin survival in E2 group was significantly longer(P
