Effect of bFGF on cyclin D1 and GADD153 expression in human ovarian cancer CAOV3 cells
- VernacularTitle:碱性成纤维细胞生长因子对卵巢癌CAOV3细胞cyclin D1及GADD153表达的影响
- Author:
Liping YE
;
Youfeng WEN
;
Haiqi NIE
;
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Cyclin D1;
Growth arrest and DNA damage inducible gene 153;
Ovarian neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effect of basic fibroblast growth factor(bFGF)on the expression of cyclin D1,growth arrest,DNA damage inducible gene 153(GADD153),and its roles involved in cell cycle regulation and DNA repair in starvation-induced ovarian cancer CAOV3 cells apoptosis.METHODS:Apoptosis of ovarian cancer CAOV3 cells was induced by serum-free culture(starvation).After bFGF treatment,the cell proliferation rate,cell cycle and apoptosis were determined by MTT,FACS analysis and agarose electrophoresis,respectively.The expression of c-Fos,c-Jun and cyclin D1,GADD153 were detected by Western blotting.RESULTS:bFGF increased the cell proliferation and prevented starvation-induced cell apoptosis.In a time-dependent manner,bFGF induced the expression of c-Fos,c-Jun and cyclin D1 and inhibited GADD153.CONCLUSION:bFGF plays a critical role in anti-apoptosis and the proliferation in human ovarian cancer by upregulating the expression of c-Fos,c-Jun and cyclin D1 and inhibiting GADD153.
