Influence of IFN-? combined with M-pred on human embryonic lung fibroblasts
- VernacularTitle:?干扰素与甲强龙合用对人胚肺成纤维细胞的影响
- Author:
Xuemei LI
;
Lidong ZHAI
;
Zhigang CAI
;
Yuzhen SHI
;
Yunli YAN
;
Xixin YAN
;
Yali WU
- Publication Type:Journal Article
- Keywords:
Lung;
Fibroblasts;
Interferon ?;
Methylprednisolone;
Transforming growth factor beta;
Collagen
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To look for a better method to deal with interstitial lung disease,interferon-gamma(IFN-?)combined with methylprednisolone(M-pred)to influence human embryonic lung fibroblast on proliferation,collagen synthesis and the expression of transforming growth factor-?1(TGF-?1)protein and mRNA were investigated.METHODS:Exponentially growing cells were preincubated for 48 h before harvested.The microculture tetrazolium(MTT)assay was used to measure the inhibition ratios of M-pred combined with different concentrations of IFN-?.The expression of proliferation cell nuclear antigen(PCNA)was detected by immunocytochemical analysis.Hydroxyproline kit was adopted to detect collagen synthesis.The expressions of TGF-?1 mRNA and protein were detected respectively by RT-PCR and Western blotting.RESULTS:Methylprednisolone,IFN-? as well as the combination of methylprednisolone and IFN-? inhibited the proliferation of HELF and the expression of PCNA in comparison with control group(P
