Effects of Brilliant Blue G on Serum Tumor Necrosis Factor-alpha Levels and Depression-like Behavior in Mice after Lipopolysaccharide Administration.
- Author:
Min MA
1
;
Qian REN
;
Ji Chun ZHANG
;
Kenji HASHIMOTO
Author Information
1. Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba, Japan. hashimoto@faculty.chiba-u.jp
- Publication Type:Original Article
- Keywords:
Coomassie Brilliant Blue;
Cytokine;
Depression;
Inflammation;
Purinergic P2X7 receptors;
Tumor necrosis factor-alpha
- MeSH:
Adenosine Triphosphate;
Animals;
Depression;
Inflammation;
Mice*;
Physical Exertion;
Receptors, Purinergic P2X7;
Tumor Necrosis Factor-alpha*
- From:Clinical Psychopharmacology and Neuroscience
2014;12(1):31-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Accumulating evidence suggests that inflammation plays a role in the pathophysiology of major depression. The adenosine triphosphate (ATP)-sensitive P2X7 receptor (P2X7R) plays a crucial role in microglial activation caused by inflammation. The dye brilliant blue G (BBG) is a P2X7R antagonist. This study examined whether BBG shows antidepressant effects in an inflammation-induced model of depression. METHODS: We examined the effects of BBG (12.5, 25, or 50 mg/kg) on serum tumor necrosis factor-alpha (TNF-alpha) levels after administering the bacterial endotoxin lipopolysaccharide (LPS; 0.5 mg/kg) and the effects of BBG (50 mg/kg) on depression-like behavior in the tail-suspension test (TST) and forced swimming test (FST). RESULTS: Pretreatment with BBG (12.5, 25, or 50 mg/kg) significantly blocked the increase in serum TNF-alpha levels after a single dose of LPS (0.5 mg/kg). Furthermore, BBG (50 mg/kg) significantly attenuated the increase in immobility time in the TST and FST after LPS (0.5 mg/kg) administration. CONCLUSION: The results suggest that BBG has anti-inflammatory and antidepressant effects in mice after LPS administration. Therefore, P2X7R antagonists are potential therapeutic drugs for inflammation-related major depression.
