Influencing Factors for Genomic DNA Extraction of Paeonia Suffruticosa
- VernacularTitle:牡丹皮基因组DNA提取的影响因素研究
- Author:
Gang XU
;
Chao YU
;
Hua ZHAO
- Publication Type:Journal Article
- Keywords:
Root Bark;
Genomic DNA;
Extraction method;
Modified CTAB method;
Molecular markers
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the influencing factors for the extraction of the genomic DNA from Paeonia Suffruticosa.METHODS:Taking Paeonia Suffruticosa(root bark of Chinese medicinal herb) as material to investigate the influencing factors including concentrations of the NaCl and beta-mercaptoethanol,temperature and time of water bath,RNaseA,PCR(polymerase chain reaction) system etc in the buffer solution on the basis of modified CTAB method.RESULTS:The DNA obtained by modified CTAB method was pure,integrated,with the value of A260/A280 ranged from 1.8 to 2.0,the ampl-ified bands of PCR were clear and bright,which lay a solid foundation for the following molecular biology experiments.CONCLUSION:The modified CTAB method is economical,rapid and efficient,and it can be served as an extraction of genomic DNA from root bark Chinese medicinal herb as well as a theoretical basis for full scale production.
