Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Ginsenoside Re in Huoxue Tongluo Tablets by HPLC
- VernacularTitle:HPLC法测定活血通络片中三七皂苷R_1、人参皂苷Rg_1和Re的含量
- Author:
Yufa YAO
- Publication Type:Journal Article
- Keywords:
Huoxue tongluo tablets;
HPLC;
Notoginsenoside R1;
Ginsenoside Rg1;
Ginsenoside Re;
Content determination
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the determination of effective components in Huoxue tongluo tablets by HPLC.METHODS:The contents of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re were simultaneously determined by a HPLC on Agilent C18(150 mm?4.6 mm,5 ?m)column,with mobile phase consisted of acetonitrile-0.05%H3PO4(gradient elution) at a flow rate of 1.0 mL?min-1 and a detection wavelength of 203 nm.RESULTS:The linear ranges of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re were 0.38～3.80 ?g(r=0.999 6),0.94～9.40 ?g(r= 0.999 5),0.15～1.50 ?g(r=0.999 8),respectively,their average recoveries were 99.23%,97.56%and 107.32%,respectively,and the corresponding RSD were 4.35%,4.94% and 4.77%,respectively.CONCLUSION:The results indicate that the HPLC method is simple,feasible and reproducible,and it is applicable for the quality control of Huoxue tongluo tablets.
