Effect of Buyang Huanwu decoction(补阳还五汤) on nuclear factor-kB and nitric oxide synthase expression in aged rats with focal cerebral ischemia/reperfusion injury
- VernacularTitle:补阳还五汤对老龄大鼠脑缺血/再灌注核转录因子-?B和一氧化氮合酶的影响
- Author:
Jianfeng GAO
- Publication Type:Journal Article
- Keywords:
Buyang huanwu decoction;
aged;
cerebral ischemia/reperfusion injury;
nuclear factor-?B;
heat shock protein 70;
nitric oxide synthase
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study the protective mechanism of Buyang Huanwu decoction(补阳还五汤) on brain damage after focal cerebral ischemia/reperfusion(I/R)in aged rats from the expression changes of nuclear factor-?B(NF-?B),heat shock protein 70(HSP70)and nitric oxide synthase(NOS).Methods A focal cerebral ischemia model was established by middle cerebral artery occlusion(MCAO),and the models were duplicated.Ninety-six aged rats were randomly divided into four groups:sham-operated group,model group,nimodipine group(6 mg?kg~(-1)?d~(-1)),Buyang Huanwu decoction group(0.9g?kg~(-1)?d~(-1)).The ischemia(I)was performed for 3 hours and reperfusion(R),for 1,3,6 and 12 days.The effects of Buyang Huanwu decoction on the nervous dysfunction score,the water content of cerebral constitution and the expressions of NF-?B,HSP70 and NOS were observed at different time points of reperfusion.Results The nervous dysfunction score,the water content of cerebral constitution,the expressions of NF-?B,HSP70, endothelial nitric oxide synthase(eNOS),neuronal nitric oxide synthase(nNOS),and inducible nitric oxide synthase(iNOS)in the model group were higher than those of the sham-operated group(P
