Determination of Serum Concentration of Phenobarbital by RP-HPLC
- VernacularTitle:RP-HPLC法测定人血浆中苯巴比妥的浓度
- Author:
Bifeng LI
;
Huiping FENG
;
Jinrong JIA
;
Jing WANG
;
Qingqing CHEN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Phenobarbital;
Serum drug concentration
- From:
China Pharmacy
2007;0(26):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the concentration of phenobarbital(PBB) in serum by RP-HPLC.METHODS: The analytical column was C18.The mobile phase consisted of methanol-water(44∶56) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 205 nm and the temperature of column was set at 35℃.RESULTS: A good linear relationship was obtained for PBB at a concentration range from 5.08 ?g?mL-1 to 63.50 ?g?mL-1(r=0.999 9).The mean relative recovery was 99.75% and the mean extraction recovery was 96.34%.The intra-day RSD and the inter-day RSD were all less than 4%.CONCLUSION: This method is simple,rapid,accurate,and suitable for therapeutic drug monitoring.
