The Expression of Aquaporin-2,3,4 in Rats' Endolymphatic Sac and Kidney
- VernacularTitle:水通道蛋白2在内淋巴囊上皮和肾脏中表达的研究
- Author:
Ting CHENG
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
Aquaporins;
Endolymphatic sac;
Kidney;
Anti-diuretic hormone(AVP);
[deamino-Cys~1,D-Arg~8]-Vasopressin(DDAVP);
Immunohistochmistry;
Fluoroimmunoassay;
Rat
- From:
Journal of Audiology and Speech Pathology
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm the expression of Aquaporin2,3,4(AQP2,3,4,water channel protein) in rats'endolymphatic sacs(ES)and kidneys and to investigate and to compare the effects of anti-diuretic hormone(AVP) and DDAVP([deamino-Cys1,D-Arg8]-Vasopressin,V2-receptor agonist)on the expression of AQP2 in rats' ESs and kidneys.Methods 30 healthy Swards white rats were divided into the negative control,AVP group and dDAVP group respectively.The animals were cardiacally perfused.The temporal bones and kidneys were taken out and sectioned by means of the paraffin-embedded technique.The sections of ESs were labeled with fluorescent antibody by immunohistochemical method,and the kidneys' with avidin-biotin-peroxidase complex method(ABC).The expressions of AQP-2 were confirmed in the ESs of the rats while the different effects of the AVP and DDAVP on the ESs and the kidneys were observed.The slides used were analyzed by the image-analyzer and the subsequent data were statistically studied.Results In the cytomembrane and cytoplasm of ESs' epithelia,the constant and clear fluorescent reaction could be observed in the normal control group with the first antibody of AQP2.Significant feeble fluorescent reaction of the first antibody of AQP-2 was revealed in AVP group and DDAVP group.In comparison with the control group,significant stain was found in AVP group and DDAVP group.Noted were also lower gray(P
