Effects of puerarin on heme oxygenase-1/carbon monoxide system in lung ischemia-reperfusion injury
- VernacularTitle:肺缺血-再灌注损伤时HO-1/CO系统的变化及葛根素对其的影响
- Author:
Xiaoxiao QIU
;
Wantie WANG
;
Yongyue DAI
;
Zhengjie XU
;
Xiwen CHEN
;
Zhouxi FANG
- Publication Type:Journal Article
- Keywords:
Lung;
Reperfusion injury;
Heme oxygenase;
Carbon monoxide;
Puerarin
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the variations of heme oxygenase-1/carbon monoxide system in lung ischemia-reperfusion injury and effects of puerarin on the system.METHODS: The unilateral lung ischemia-reperfusion model was replicated in vivo.Rabbits were randomly divided into three groups(n=10 in each): control group,ischemia-reperfusion(I-R) group and puerarin group.The blood specimens collected at times before ischemia,ischemia 1 h,reperfusion 1 h,3 h and 5 h were tested for the contents of carboxyhemoglobin(COHb) and cyclic guanosine monophosphate(cGMP).The lung tissues sampled at 5 h after reperfusion were assayed for wet/dry weight ratio(W/T),the injured alveoli rate(IAR) and the activity of HO-1.The changes of ultrastructure were observed under electron microscope.The tissue slides were also stained by immunohistochemistry(IHC) and in situ hybridization(ISH) to detect the expression of HO-1.RESULTS: The plasma content of COHb and cGMP in I-R and puerarin group increased in a time-dependent manner after I-R compared with control group,but the increment in puerarin group was higher than that in I-R group(P
