Effect of heparin,IGFBP-4 and IGF-1 on glucose metabolism and function in fibroblasts
- VernacularTitle:肝素、IGFBP-4及IGF-1对肺成纤维细胞糖代谢及功能的影响
- Author:
Yongxin QIN
;
Xianyao WAN
;
Baiyi CHEN
- Publication Type:Journal Article
- Keywords:
Heparin;
Lung;
Fibroblasts;
Glucose metabolism;
Insulin-like growth factor 1;
Insulin-like growth-factor-binding proteins
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibitory effect of IGF binding protein-4(IGFBP-4),especially in corporation with heparin on the pathopoiesis of insulin-like growth factor-1(IGF-1) in alveolitis and fibrosis.METHODS: The diploid human embryonic lung(HEL) fibroblasts were incubated respectively with control,100 ?g/L IGF-1,100 ?g/L IGF-1+100 ?g/L IGFBP-4,100 ?g/L IGF-1+200 ?g/L IGFBP-4,100 ?g/L IGF-1+100 ?g/L IGFBP-4+100 ?g/L heparin,100 ?g/L IGF-1 + 100 ?g/L IGFBP-4+200 ?g/L heparin,100 ?g/L IGF-1+100 ?g/L heparin,100 ?g/L IGF-1+200 ?g/L heparin for 24 h.Then the content of glucose transporter-4(GLUT-4),hexokinase-2(HK-2),collagen-4 and elastin were detected,respectively.RESULTS: Compared with control group,HK-2,GLUT-4,elastin and collagen-4 expressed in IGF-1 group were increased obviously.The expression in the group of IGFBP-4 plus IGF-1 was more than that in IGF-1 group.However,all expression was depressed strikingly when heparin was added.CONCLUSION:(1) IGF-1 apparently stimulates HK-2,GLUT-4,elastin and collagen-IV secretions from lung fibroblasts.(2) The intact IGFBP-4 associated with heparin can inhibit the pathopoiesis of IGF-1.
