Effect of fibrin on expression of interleukin-6 in rat brain vascular endothelial cells
- VernacularTitle:纤维蛋白对大鼠脑血管内皮细胞白细胞介素6表达的影响
- Author:
Zheng XUE
;
Suming ZHANG
- Publication Type:Journal Article
- Keywords:
Fibrin;
Brain;
Vascular endothelial cells;
Interleukin-6;
Rats
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the kinetics of interleukin-6(IL-6) protein and mRNA expressions in the co-culture of rat brain vascular endothelial cells(VECs) with fibrin.METHODS: The IL-6 concentration were measured by rat IL-6 ELISA kit and the IL-6 mRNA was measured by real-time PCR after development an in vitro model of in situ fibrin polymerization on rat brain vascular endothelial cells.RESULTS: Fibrin induced IL-6 expression.The IL-6 antigen concentration increased significantly in 1.0 g/L media group compared to the low dose fibrin group or control media group(P
