Effect of siRNA for survivin gene on growth and apoptosis in A549 cells
- VernacularTitle:Survivin siRNA对人肺腺癌A549细胞增殖影响实验研究
- Author:
Qigang ZHAO
;
Lei SI
;
Xiaoxia TIAN
;
Peng LI
- Publication Type:Journal Article
- Keywords:
Genes,survivin;
Lung neoplasms;
A549 cells;
Gene therapy
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the influence of lentiviral vectors expressing siRNA for survivin gene knockdown in A549 cells,sequentially as tools to explore the molecule pathogenesis and new gene therapy of lung adenocarcinoma.METHODS: The lentiviral vectors,which express survivin siRNA,were constructed and transfected into A549 cell strain.The titers of the lentiviruses were determined by 293T cells.The expressions of survivin and caspase-3 were detected by Western blotting and RT-PCR.The cell cycle and cell growth of A549 cells were examined by MTT and FCM.RESULTS: The expression of survivin was suppressed effectively by siRNA targeting survivin.The expression of survivin mRNA decreased by 97%.The expression of survivin protein decreased by 94%.The rate of cell growth was decreased.The G1 phase cells were increased,whereas S phase cells were decreased.CONCLUSION: The lentivirus vectors expressing siRNA for survivin can significantly inhibit gene expression and the cell growth,and markedly induce the apoptosis.It is hopeful to be a new gene therapy of lung adenocarcinoma.
