The relationship between pancreatic iNOSmRNA expression and gut permeability and the effect of salviae miltiorrhizae on the change in gut permeability in rats with severe acute pancreatitis
- VernacularTitle:急性胰腺炎胰腺iNOS mRNA的表达与肠壁通透性的关系及丹参对其的影响
- Author:
Chengxian SHI
;
Yuxiang LI
;
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,Acute Necrotizing/pathol;
Inducible Nitris Oxide Synthase,;
Situ Hybridization;
Intestinal Permeability;
Salvia Miltiorrhiza
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between inducible nitric oxide synthase(iNOS)mRNA expression of pancreatic tissues and intestinal permeability,and the effect of salviae miltiorrhizae on the change in gut permeability in rats with severe acute pancreatitis(SAP).Methods All rats were randomly divided into three groups:Sham operation(group C,n=15),SAP group(group SAP,n=15),and salviae miltiorrhizae therapeutic group(group T,n=15).At 24h After operation,the iNOSmRNA expressions of pancreas were determined by in situ hybridization(ISH),and blood amylase(AMY),nitric oxide(NO) and lipopolysaccharide(LPS) were examined.The histopathologic changes of pancreas and ileum were observed.Intestinal permeability was measured by means of albumin clearance(AC)of 125I-labeled rat serum albumin.Results The iNOSmRNA expressions of pancreas in group SAP were markedly increased in comparison with group T(P
