Role of expression of X-linked inhibitor of apoptosis protein in polymorphonuclear neutrophils during acute pancreatitis
- VernacularTitle:急性胰腺炎外周血中性粒细胞凋亡及XIAP表达的意义
- Author:
Liqiu LIAO
;
Yixiong LI
;
Yangyang OU
;
Xinsheng LU
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
PMN;
Apoptosis;
XIAP
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of apoptosis and the expression of X-linked inhibitor of apoptosis protein(XIAP) in polymorphonuclear neutrophils(PMNs) during acute pancreatitis(AP).Methods Blood from normal control(NC,n=15),mild acute pancreatitis(MAP,n=15) and severe acute pancreatitis(SAP,n=15) were collected.PMNs apoptosis was detected by flow cytometry.PMNs were isolated from each group and XIAPmRNA and protein levels were assessed by RT-PCR and Western Blotting.Results PMNs apoptosis in SAP group was(2.15?0.40)%,MAP group was(4.16?0.14)%,NC group was(4.31?0.12)%.PMNs apoptosis rate in SAP and MAP groups was decreased compared to NC group(P
