Optimization of the Microwave-assisted Extraction of Total Organic Acids from Isatis indigotica by Or-thogonal Test
- VernacularTitle:正交试验优选微波法提取板蓝根总有机酸的工艺
- Author:
Liming LEI
;
Yunkai JIANG
;
Qian GAO
;
Shimei LIU
- Publication Type:Journal Article
- Keywords:
Isatis indigotica;
Microwave-assisted extraction;
Total organic acids;
Orthogonal test
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the microwave-assisted extraction of total organic acids from Isatis indigotica. METHODS: With the dissolution rate of total organic acids as indexes, the content of total organic acids was determiend by acid-base titration and the three main factors including volume of solvent, microwave power and extraction time in the extraction process were optimized by orthogonal test. RESULTS: The optimal extraction process is to add 15 times amount of water and extract at a microwave power of 522 W for 20 minutes. Under the above condition, the extraction rate was 4.83%, close to the extraction yield achieved by water decoction for 2 h. CONCLUSION: The microwave-assisted extraction of total organic acids from Radix Isatidis is characterized by rapid, simple, efficient and energy-saving etc, thus deserving to be be popularized.
