Determination of the Content of Stilbene Glycoside in Zhipingkang Misture by HPLC
- VernacularTitle:HPLC法测定脂平康合剂中二苯乙烯苷的含量
- Author:
Xianghua QUAN
;
Huiming WANG
;
Xianghong SUN
;
Hong LI
;
Chuan WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Zhipingkang misture;
Stilbene glycoside;
Content determination
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of Stillbene glycoside (THPG) in Zhipingk-ang misture.METHODS:The chromatographic column was Hypersil C18(250 mm?4.6 mm,5 ?m).The mobile phase consisted of acetonitribl-water(25∶75) at a flow rate of 1.0 mL?min-1 with detection wavelength set at 320 nm. RESULTS: There was a good linear relationship within the concentration range of 0.5～8.0 ?g for THPG. The average recovery of THPG was 100.03%(RSD=1.07, n=6).CONCLUSION: The adoptd method is accurate, reproducible and suitable for the quality con-trol of Zhipingkang misture.
