Determination of Oleanolic Acid in Ziyin shugan Granule by RP-HPLC
- VernacularTitle:RP-HPLC法测定滋阴舒肝颗粒中齐墩果酸的含量
- Author:
Lijiang LIAO
;
Yingtong ZENG
;
Haiyong LIANG
- Publication Type:Journal Article
- Keywords:
Ziyin shugan granule;
Oleanolic acid;
RP-HPLC;
Content determination
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method to determine the content of Oleanolic acid in Ziyin shug-an granule. METHODS: Kromasil C18 column(250 mm?4.6 mm,5 ?m)was used as chromatographic column,and the mobile phrase consisted of methanol-0.2% phosphoric acid(80∶20) at a flow rate of 1.0 mL?min-1 and the det-ection wavelength was set at 208 nm,and the column temperature was maintained at room temperature.RESULTS:The calibration curve of Oleanolic acid was in good linearity over the range of 0.237～4.740 ?g(r=0.999 8).The average recovery of Oleanolic acid was 98.84% with RSD at 1.19%(n=6).CONCLUSION:The method is simple,feasible and reproducible, and can be used for the quality control of Ziyin shugan granule.
