Inhibitory effect and induction of apoptosis of caffeic acid Ge on growth of U14 in mice
- VernacularTitle:咖啡酸锗对小鼠U14瘤的抑制作用及其诱导肿瘤细胞凋亡的机制研究
- Author:
Yueyan HUANG
;
Chun XIAO
;
Mingjuan WU
;
Yangfan YU
;
Chunhua LIU
;
Youhui XU
- Publication Type:Journal Article
- Keywords:
Caffeic acids;
Ge;
U14 cells;
Apoptosis;
Genes,bax;
Genes,bcl-2;
Protein Bax;
Protein Bcl-2
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the antitumor effect of caffeic acid Ge on U14 tumor bearing mice.METHODS:The tumor inhibitory ratios of caffeic acid Ge on the growth of U14 in mice was observed.Apoptosis morphological transformation of U14 cells induced by caffeic acid Ge was detected by electronic scan microscope and MG-P staining.Alteration of cell cycle was analyzed by flow cytometry.Apoptosis-related protein levels of Bax and Bcl-2 were determined by immunity histochemistry technology.MTT assay was applied to study the antitumor activities of caffeic acid Ge in U14 cell lines in vitro.RESULTS:Tumor inhibitory rates in caffeic-acid Ge groups were 38.50%,47.17% and 64.02%(from low dose to high dose)(P
