Detection of associated genes in human lung squamous cell carcinoma in early stage with tumor cDNA microarray
- VernacularTitle:应用肿瘤基因芯片筛选早期肺鳞癌相关基因
- Author:
Li LIU
;
Qin YANG
;
Wei HUANG
;
Qian WU
;
Ting YANG
;
Chang HE
;
Peng RAN
;
Feng LING
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Gene expression profiling;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the gene expression profile of human lung squamous cell carcinoma in early stage.METHODS:The mRNA was extracted from cancer tissue and normal lung tissue.The mixed probes were labeled and hybridized with chip containing 480 carcinoma related genes,then analyzed by SuperArray Image software to study the gene expression patterns in lung squamous cell carcinoma.RESULTS:192 genes associated with lung squamous cell carcinogenesis were found,which were grouped into transporter,adhesion molecules,cytoskeleton proteins,transcription regulator,genes associated with metabolism and immune response according to functions.127 gens were positive to lung squamous cell carcinogenesis and 65 genes were negative to lung squamous cell carcinogenesis.CONCLUSION:The screen of gene expression profile of human lung squamous cell carcinoma provides valuable information for the research of molecular mechanism of the lung squamous cell carcinogenesis.
