Change of mitochondria during apoptosis in Jurkat cells induced by arsenic trioxide
- VernacularTitle:三氧化二砷诱导Jurkat细胞凋亡过程中线粒体的变化
- Author:
Fang HE
;
Yaoying ZENG
;
Tong WANG
;
Jingxian ZHAO
- Publication Type:Journal Article
- Keywords:
As2O3;
Jurkat cells;
Apoptosis;
Mitochondria
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the changes of mitochondria during apoptosis in Jurkat cells induced by arsenic oxide(As2O3).METHODS:By treated with 4?10-6 mol/L As2O3,apoptosis and necrosis of Jurkat cells were assessed by annexin V-FITC/PI double staining flowcytometry.Mitochondrial mass and its membrane potential(△?m)was measured by NAO/PI and DiOC6(3)/PI staining,respectively.Free radical formation was detected by DCFDA staining.RESULTS:After 48 h of As2O3 treatment,the rates of early apoptotic Jurkat cells in As2O3 and control groups were(18.98?1.40)% and(5.17?0.80)%,respectively(P
