Effect of Procyanidins on Caspase-9 mRNA and Cytochrome C Release in Mitochondrion of Hippocampal of Rats' Brain after Ischemic Reperfusion Injury
- VernacularTitle:原花青素对大鼠局灶性脑缺血再灌注损伤后海马线粒体CytC的释放和Caspase-9的影响研究
- Author:
Yijun ZHANG
;
Wenming MA
;
Zhengping WANG
- Publication Type:Journal Article
- Keywords:
Procyanidins;
Rat;
Mitochondrion;
Cytochrome C;
Caspase-9 mRNA
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the mechanism of procyanidins on mitochondrion injury caused by cerebral ischemia reperfusion(IR) in rats.METHODS:Forty-eight Wistar rats were randomly separated into 4 groups including sham operation group,IR model group,procyanidins pretreatment high and low doses groups(400,40 mg?kg-1).After medication for 30 days,the reperfusion model following focal cerebral ischemia in rats was made by thread embolish of MCA,and the rats were put to death 2 h later for sampling of brain tissues.The expression of cytochrome C protein was detected by immunofluorescence method and that of caspase-9 mRNA was detected by RT-PCR technique.RESULTS:There were significantly more cytochrome C and less caspase-9 mRNA in procyanidins pretreatment group than in IR model group(P
