Determination of Chicoric Acid in Three Different Pharmaceutical Dosage Forms by RP-HPLC
- VernacularTitle:RP-HPLC法测定3种不同药物剂型中菊苣酸的含量
- Author:
Xianmin MENG
;
Rong LIU
;
Ping DONG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Chicoric acid;
Content determination;
Oral liquid;
Tablet;
Capsule
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the contents of chicoric acid in three different pharmaceutical dosage forms by RP-HPLC.METHODS:Samples were determined on VP-ODS C18,with the mobile phase consisted of methanol-1% acetic acid-tetrahydrofuran(33∶62∶5) with flow rate at 1.0 mL?min-1,UV detection wavelength at 327 nm,column temperature at 30 ℃ and sample size at 20 ?L.RESULTS:The linear range of chicoric acid was 0.398 72～3.987 2 ?g(r=0.999 8).The average recoveries of the oral liquid,capsules,and tablets were 99.85%(RSD=0.98%),102.50%(RSD=1.84%),and 100.50%(RSD=1.69%),respectively.CONCLUSION:This method is accurate,reproducible,specific,and suitable for the content determination of chicoric acid.
