Tachpleus Amebocyte Lysate Test for Bacterial Endotoxins in Cardioplegia Solution
- VernacularTitle:停跳液细菌内毒素检查
- Author:
Dejian CHEN
- Publication Type:Journal Article
- Keywords:
Cardioplegia solution;
Bacterial endotoxin;
Tachypleus amebocyte lysate test
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method of assaying bacterial endotoxin in cardioplegia solution.METHODS:The tachpleus amebocyte lysate(TAL) test for bacterial endotoxin in cardioplegia solution was performed in accordance with Chinese Pharmacopoeia(2005 Edition,Volume Two).RESULTS:The 8-fold diluents of cardioplegia solution did not interfere with the test for bacterial endotoxins,with Et=0.5 Es～2 Es.CONCLUSION:It is feasible to detect bacterial endotoxins in cardioplegia solution with TAL at a sensitivity of 0.06 EU?mL-1.
