Optimization of the Separation and Purification Process of Astragaloside Ⅳ by Orthogonal Experiment
- VernacularTitle:正交试验优选黄芪甲苷的分离纯化工艺
- Author:
Xiaofeng ZHENG
- Publication Type:Journal Article
- Keywords:
Astragaloside Ⅳ;
Macroreticular resins;
Separation and purification;
Orthogonal experiment
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the technologic conditions for separation and purification process of Astragaloside Ⅳ using macroreticular resins.METHODS:The optimal extraction process and purification technology using macroreticular resins were investigated by orthogonal experiment design with extract yield and content of Astragaloside Ⅳ as evaluation indexes,and the influencing factors were also investigated.RESULTS:The optimal conditions for extraction were as follows:sixfold amount of water was added to extract Astragaloside Ⅳ for 3 times with 1 h each time.The isolation and purification of Astragaloside Ⅳ were influenced by relative density,elution dosage and velocity.CONCLUSION:The established optimal technologic conditions for the extraction,separation and purification of Astragaloside Ⅳ using macroreticular resins proved to be feasible.
