Determination of Baicalin in Xiaoyan Qingre Capsules by HPLC
- VernacularTitle:HPLC法测定消炎清热胶囊中黄芩苷的含量
- Author:
Liwei YANG
;
Fei LONG
;
Shuxiong XIAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Baicalin;
Xiaoyan qingre capsules;
Content determination
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for the determination of Baicalin in Xiaoyan Qingre capsules.METHODS:The separation of sample was performed on Thermo ODS-2 Hypersil(150 mm?4.6 mm,5 ?m).The mobile phase was methanol-0.1% phosphoric acid(43∶57).The detection wavelength was 277 nm,and the temperature of column was 30 ℃.RESULTS:The linear range of Baicalin was 0.217 8～3.267 0 ?g(r=0.999 9).The average recovery was 100.73%(RSD=2.23%,n=6).CONCLUSION:The method is sensitive,accurate,reproducible,and suitable for the qual-ity control of Xiaoyan qingre capsule.
