Determination of Paeoniflorin in Xuefu Zhuyu Liquor by RP-HPLC
- VernacularTitle:RP-HPLC法测定血府逐瘀口服液中芍药苷的含量
- Author:
Zhonglan YANG
- Publication Type:Journal Article
- Keywords:
Xuefu zhuyu liquor;
Paeoniflorin;
RP-HPLC;
Content determination
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an RP-HPLC method for the determination of Paeoniflorin in Xuefu zhuyu liquor.METHODS:A SunFireTM C18(250 mm?4.6 mm,5 ?m) column was used with the mobile phase consisted of acetonitrile-1% glacial acetic acid-methanol(5∶24∶71) at a flow rate of 1.0 mL?min-1.The temperature of column was 25 ℃ and the dete-ction wavelength was set at 240 nm.RESULTS:Paeoniflorin showed a good linear relationship at a range of 0.038 4～0.307 2mg?mL-1(r=0.999 6),and the average recovery rate was 100.26%(RSD=1.36%,n=9).CONCLUSION:The method is simple and accurate,and it can be used for the quality control of Xuefu zhuyu liquor.
