Protective effect of safflower solution on warm ischemia reperfusion injury of liver
- VernacularTitle:红花注射液对肝热缺血再灌注损伤保护作用的研究
- Author:
Xin QI
;
Xiangqian CHE
;
Chao WANG
;
Zhijun HE
- Publication Type:Journal Article
- Keywords:
Liver/blood supply;
Ischemia Reperfusion Injury;
Ischemic Preconditioning;
Safflower
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect and mechanism of safflower solution on warm ischemia reperfusion injury(IRI) of liver in rats.Methods Male Sprague-Dawley rats were randomly divided as 4 groups: Group S(sham group);group I/R(IRI group);group IPC(ischemic preconditioning group);group SPC(safflower solution preconditioning group).The rats were sacrificed at 24 h after reperfusion,serum levels of ALT and AST were measured and HE staining of liver tissues were made to detect rat liver histological changes and grade liver IRI(Suzuki score);apoptosis were monitored by TUNEL;TNF-?,MIP-2 and ICAM-1 mRNA of liver were measured by RT-PCR;NF-?B gene products of liver were detected by Western blotting.The mean value of all the assay tests was compared.Results After 24 h reperfusion,compared with group I/R,the liver function(ALT and AST) in group SPC and group IPC was significantly improved(P
