Effect and mechanism of Querecetin treatment of transplanted hepatic cancer in nude mice
- VernacularTitle:Quercetin治疗裸鼠移植人肝癌的作用及机制
- Author:
Xi HUANG
;
Jihong ZHANG
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocellular;
Quercetin;
Genes,bax
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of 1,4,5-trisphosphate inositol(IP3) and bax gene expression in inhibiting transplanted hepatocellular carcinoma of nude mice by quercetin.Methods After animals with hepatocellular carcinoma were treated with quercetin 1mg/kg/d(ip)for 3 weeks,the volume and weight of tumor was measured,and IP3,Bax mRNA,and Bax protein were assayed by IP3- Birtrak assay,RT-PCR,and Western blotting,respectively;and compard with coutrol group.Results The tumor volume and weight of animals treated with quercetin were lower than those of control[(15.8?10.1) mm3 vs.(52.3?26.5 mm3;(44.8?10.4) mg vs.(91.3?31.4) mg],IP3 content was lower than that of control[(15.9?2.8)pmol/mg protein vs(35.3?6.6)pmol/mg protein],Bax mRNA expression was not significantly different between the 2 groups[RI which was the gray degree multiply area of bax / the gray degree multiply area of ?-actin(0.64?0.12) vs.(0.56?0.15)],Bax mRNA expression was higher in group treated with quercetin than that of control[(3.16?0.95) vs.(1.37?0.48)].Conclusions Quercetin can inhibit growth of transplanted hepatocellular carcinoma of nude mice by reducing IP3 production and bax protein expression.
