Role of ROS and energy metabolism dysfunction in hepatocyte adipose degeneration in vitro
- VernacularTitle:活性氧及能量代谢障碍在体外培养肝细胞脂肪变性中的作用
- Author:
Chaofeng HU
;
Daxing LU
;
Liping SUN
- Publication Type:Journal Article
- Keywords:
Hepatocytes;
Adipose degeneration;
Reactive oxygen species;
Mitochondrial membrane potential;
Energy metabolism
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the role of reactive oxygen species(ROS)and energy metabolism dysfunction in hepatocyte adipose degeneration induced by alcohol and calf serum(CS).METHODS:The growing L02 cells were treated with different concentrations of alcohol.To screen the proper concentration of alcohol,the proliferation of cells was measured by MTT.Lipid droplets in the cells were observed through oil red staining.Triglyceride(TG)content was detected with analyzed kit.The level of ROS and changes of mitochondrial membrane potential(??m)in cells were tested by flow cytometry.Reversed-phase high performance liquid chromatography(RP-HPLC)was applied to assay the cellular ATP content.RESULTS:Lipid droplets were observed under light microscope in the cells treated with 2% alcohol and 50% CS(A+CS)for 36 h.Compared to control group,the cellular TG and ROS levels in model group markedly increased while ??m and ATP content in cells significantly decreased(P
