Production and cytotoxicity of the reactive oxygen species induced by diallyl trisulfide in human myeloid leukemia HL-60 cells
- VernacularTitle:二烯丙基三硫诱导人髓样白血病HL-60细胞活性氧产生及其细胞毒性作用
- Author:
Youhua WU
;
Xiaoxiao CAO
;
Mengxia ZHANG
;
Zhizhen TIAN
;
Xiaoyong LEI
;
Jian TU
;
Hongmei LUO
;
Shengsong TANG
- Publication Type:Journal Article
- Keywords:
Diallyl trisulfide;
NADPH oxidase;
Reactive oxygen species;
Malondialdehyde;
HL-60 cells
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the production and cytotoxicity of the reactive oxygen species(ROS)induced by diallyl trisulfide(DATS)in HL-60 cells.METHODS:HL-60 cells were either treated with various doses of DATS alone,or DATS combination with apocynin,a specific NADPH oxidase inhibitor,or with antioxidant N-acetyl-L-cysteine(NAC)for 0,1,3,6,12 and 24 h,respectively.The intracellular ROS level was measured by flow cytometry.The activity of NADPH oxidase was evaluated by NBT reduction experiment.The content of both malondialdehyde(MDA)and the protein carbonyl were analyzed by spectrophotometer.RESULTS:The results from flow cytometry indicated that DATS significantly increased the intracellular ROS level in HL-60 cells(P
