Trichostatin A inhibits serum-induced p27kip1 protein degradation in vascular smooth muscle cells
- VernacularTitle:曲古菌素A抑制血清诱导血管平滑肌细胞p27kip1表达的降解
- Author:
Chen ZOU
;
Chunfang WU
;
Zhihong XU
;
Guoping LU
- Publication Type:Journal Article
- Keywords:
Trichostatin A;
Vascular smooth muscle cells;
Genes,p27kip1;
S-phase kinase-associated protein-2
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of trichostatin A(TSA)on p27kip1 gene expression in vascular smooth muscle cells.METHODS:Reverse transcription-polymerase chain reaction(RT-PCR)was used to measure the level of p27kip1 mRNA.The protein levels of p27kip1 and S-phase kinase-associated protein-2(skp2)were determined by Western blotting.20S proteasome activity was quantified by using a fluorogenic proteasome-specific substrate.RESULTS:TSA did not affect mRNA level of p27kip1 in VSMCs,but attenuated serum-induced downregulation of p27kip1 through stabilizing p27kip1 turnover.In addition,TSA decreased the expression of skp2,an F-box protein that targets p27kip1 for degradation,but had no effect on proteasome activity.CONCLUSION:TSA regulates p27kip1 expression at the post-translational level in VSMCs.
