Effects of 1,25-droxyvitamin D_3 on Nitric Oxide and Inducible Nitric Oxide Synthase in Peripheral Blood Mononu-clear Cells in Patients with Spinal Tuberculosis
- VernacularTitle:1,25-二羟维生素D_3对脊柱结核患者外周血单个核细胞的NO及iNOS的影响研究
- Author:
Song WANG
;
Chunyan DUAN
;
Qing WANG
- Publication Type:Journal Article
- Keywords:
1,25-droxyvitamin D3;
Spinal tuberculosis;
Quantitative PCR;
Nitric oxide;
Nitric oxide synthase
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of 1,25-droxyvitamin D3 on nitric oxide and nitric oxide synthase in peripheral blood mononuclear cells(PBMC)in patients with spinal tuberculosis and to explore its immunoregulation function.METHODS:Patients' PBMC were assigned to test group in which DHVD3 was added or control group in which culture medium was added,then cultured after BCG vaccine inoculation.Cultured supernatant fluid from isolated PBMC was collected at 0,3,6,9,12 days respectively for measuring of relative optical density(OD)of NO by nitric oxide kit.Total RNA extracted from the PBMC was reverse transcribed to cDNA and amplificated by fluorecent real-time quantitative PCR on the sixth day;mRNA of target gene iNOS and reference gene 3-GAPD were analyzed and compared by relative quantitative method 2-??Ct.RESULTS:OD value of NO and mRNA of iNOS in test group were all higher than in control group(P
