Improvement of the Method of Positive Control Test in Bacterial Endotoxin Test
- VernacularTitle:细菌内毒素检查法中供试品阳性对照试验方法的改进
- Author:
Zhide LI
;
Xia WANG
- Publication Type:Journal Article
- Keywords:
Bacterial endotoxin test;
Positive control test;
Improvement of method
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the method of positive control test in bacterial endotoxin test.METHODS:A new method rather than a routine method was adopted for the bacterial endotoxin test in which the tachyplens amebocyte lysate(TAL)was dissolved directly by sample solutions or its diluted solution,which was then added with 2.0 ? endotoxins solution for positive control test for the common injections including metronidazole and glucose saline etc.RESULTS:The Et values of all the test samples detected by the improved method were similar to those by the routine method,which stood at 2.0 Es～0.5 Es,suggesting that the improved method has no interfere on the bacterial endotoxin test.CONCLUSION:The method is simple and time-saving.
