Determination of the Content of Main Components in Lipoic Acid Sustained-release Tablets by HPLC
- VernacularTitle:HPLC法测定硫辛酸缓释片中主药的含量
- Author:
Liang SUN
;
Shouyao ZHANG
;
Zhongyi ZHANG
;
Aimin JI
- Publication Type:Journal Article
- Keywords:
HPLC;
Lipoic acid sustained release tablets;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of lipoic acid in lipoic acid sustained-release tablets.METHODS:HPLC was conducted on a Hypersil ODS with acetonitrile-0.05 mol?mL-1 potassium dihydrogen phosphate(45∶55,adjust pH to 2.0)as mobile phase at a flow rate of 1.0 mL?min-1.The injection volume was 10 ?L;the detection wavelength was set at 219 nm and the column temperature was 25 ℃.RESULTS:The linear range of lipoic acid was 9.99～399.70 ?g?mL-1(r=0.999)with an average recovery rate of 99.17%(RSD=0.40%);the intra-day and inter-day RSD were all less than 2.6%.CONCLUSION:The method is simple and accurate,and suitable for the determination of the content of lipoic acid in lipoic acid sustained-release tablets.
