HPLC Fingerprint of Rhizoma Belamcandae
- VernacularTitle:射干药材HPLC指纹图谱研究
- Author:
Ying CAI
- Publication Type:Journal Article
- Keywords:
Rhizoma Belamcandae;
Belamcandin;
RP-HPLC;
Fingerprint;
Canonical discriminant function;
Fisher's di-scriminant function
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the HPLC fingerprint of Rhizoma Belamcandae. METHODS: The fingerprint of Rhizoma Belamcandae was established by HPLC on Lichrospher C18(250 mm?4.6mm,5um).The mobile phase consisted of 1% acetic acid acetonitrile -1% acetic acid solution at a flow rate of 1.0 mL?min-1. The column temperature was 30 ℃.Cluster analysis was conducted with peak area as quantitative characteristic value. Canonical discriminant function and Fisher's discriminant function of the HPLC finger print of Rhizoma Belamcanda were established using stepwise classification scheme. RESULTS: The fingerprint of Rhizoma Belamcandae has been established. The discrimination and classification results by the two kinds of functions were totally consistent with each other, with corresponding rate of 100%. CONCLUSION: The two discriminant functions are of theoretical and practical value for they provide an accurate and rapid tool for the identification of the unknown samples as well as a new model for the quality control of Chinese medicines.
