Determination of Matrine in Baishan Tablets by HPLC
- VernacularTitle:HPLC法测定白山片中苦参碱的含量
- Author:
Chuntao WANG
;
Hong FU
- Publication Type:Journal Article
- Keywords:
Baishan tablets;
Matrine;
HPLC;
Content determination
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of Matrine in Baishan tablets. METHODS: The analytical column was Aminopropyl (200 mm?4.6 mm, 10 ?m) with mobile phase consisted of acetonitrile-0.5% phosphate solution - ethanol(100∶8∶10) at a flow rate of 1.0 mL?min-1. The column temperature was set at 30 ℃ and the detection wavelength was set at 220 nm. RESULTS: The linear range of Matrine was 0.27～ 8.66 ?g(r=0.999 8) and the average recovery of Matrine was 99.52 %(RSD=0.91%,n=6). CONCLUSION: The method is simple, accurate and reproducible, and it is applicable for the quality control of Baishan tablets.
