Imipenem-resistant Mechanism of Pseudomonas Aeruginosa
- VernacularTitle:铜绿假单胞菌对亚胺培南的耐药性分析及机制探讨
- Author:
Yijie ZHANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginos;
Imipenem;
Resistance;
OprD2;
Metallo-?-lactamases
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism of imipenem-resistance in clinical isolates of Pseudomonas aeruginos (PA). METHODS: 55 strains of PA had been isolated from sputum specimen in clinic and their drug sensitivity to imipenem was tested by K-B slip diffusion method. Their amount of outer membrane protein OprD2 was analyzed by SDS-PAGE with outer membrane protein profiles. Metallo-?-lactamases (MBLs) was detected by double-disk synergy test (DDST). RESULTS: 36 strains of imipenem-resistant and 19 strains of imipenem-sensitive PA isolated were studied. The amount of OprD2 showed a various degree of reduction in all resistant isolates, and the relative amount of OprD2 in resistant isolates were significantly lower than in sensitive isolates (P
