Expression of urokinase: type plasminogen activator, its receptor, and its inhibitor in gastric adenocarcinoma tissues.
10.3346/jkms.1996.11.1.33
- Author:
Seok Il HONG
1
;
In Chul PARK
;
Young Sook SON
;
Seung Hoon LEE
;
Byoung Gie KIM
;
Jong Inn LEE
;
Tai Won LEE
;
Yoon Hoh KOOK
;
Young Il MIN
;
Weon Seon HONG
Author Information
1. Laboratory of Cell Biology, Korea Cancer Center Hospital, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
gastric adenocarcinoma;
metastatic lymph nodes;
Urokonase-type plasminogen activator;
Plasminogen activator receptor;
Plasminogen activator inhibitor-1;
Northern blot analysis;
Immunohistochem
- MeSH:
Adenocarcinoma/*metabolism/pathology;
Gastric Mucosa/metabolism;
Gene Expression;
Human;
Immunoenzyme Techniques;
Lymph Nodes/metabolism/pathology;
Neoplasm Metastasis;
Plasminogen Activator Inhibitor 1/*biosynthesis/genetics;
Plasminogen Activators/*biosynthesis/genetics;
RNA, Messenger/biosynthesis;
Receptors, Cell Surface/*biosynthesis/genetics;
Stomach Neoplasms/*metabolism/pathology;
Support, Non-U.S. Gov't;
Urinary Plasminogen Activator/*biosynthesis/genetics
- From:Journal of Korean Medical Science
1996;11(1):33-37
- CountryRepublic of Korea
- Language:English
-
Abstract:
The plasminogen and plasmin system, which is mainly regulated by urokinase-type plasminogen activator (uPA), its receptor (uPAR) and its inhibitor (PAI-1), is generally believed to play a role in cancer invasion and metastasis. This study was conducted to investigate the role of uPA, uPAR and PAI-1 in the invasion and metastasis of gastric adenocarcinoma. The expression of mRNAs for uPA and PAI-1 was determined by Northern blot analysis in nine primary gastric cancer tissues, nine paired metastatic lymph nodes and normal gastric mucosa. The mRNA of uPA was not or faintly detected in normal mucosa, while the expression was increased in both primary gastric cancer tissues and metastatic lymph nodes to a similar degree. The mRNA expression for PAI-1 in the gastric cancer tissues was not different from that in the paired metastatic lymph nodes and normal mucosae. uPAR was determined by immunohistochemical staining, demonstrating that five (56%) and six (67%) out of nine primary gastric cancer tissues and nine paired metastatic lymph nodes were positive, respectively and the intensity was stronger in metastatic lymph nodes. The results support the concept that most gastric cancer cells may have an innately moderate level of uPA and uPAR, and that increase of uPAR expression can be considered to be closely associated with cancer invasion and metastasis.
