Site specific antibody of Na/Ca exchanger stimulates Na/Ca exchanger current and has cross-reaction with Ca channel and Na pump
- VernacularTitle:抗钠钙交换体特异位点抗体对心肌钠钙交换电流的激活作用及其与钙通道、钠泵的交叉反应
- Author:
Xiaojie BAI
;
Bowei WU
- Publication Type:Journal Article
- Keywords:
Sodium-calcium exchange;
Antibodies;
Calcium channels;
Sodium pump;
Heart
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
1∶640) was purified by affinity chromatography with a Mab Trap kit.The effects of the purified IgGs on the ion channel and transporter(INa/Ca,INa pump,ICa-L,Itko,Ik1,IK) at concentrations of 10 nmol/L,20 nmol/L and 40 nmol/L were observed by whole cell voltage clamp technique.RESULTS:The purified IgG enhanced INa/Ca,ICa-L and I Na/k pump in a dose-dependent manner and no significant effect on INa,Itko,Ik1 and Ik was found.CONCLUSION:The site specific antibody of Na-Ca exchanger stimulates Na-Ca exchanger current and has cross-reaction with L-type Ca channel and Na pump.
