Effects of progesterone on the maturation and immunologic function of dendritic cells from human peripheral blood
- VernacularTitle:孕酮对人树突状细胞成熟和免疫功能的影响
- Author:
Yuan SHEN
;
Yaoying ZENG
;
Jingxian ZHAO
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Progesterone;
Immunological function
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effects of progesterone(P4) on the maturation and immunologic function of dendritic cells(DCs) from human peripheral blood.METHODS:Cultured DCs were treated with P4 at doses of 10-7 mol/L and 10-6 mol/L.The morphologic changes were observed under the scanning electronic microscope.The immunophenotypes of DCs in control and treated groups were analyzed by flow cytometry.IL-10 and IL-12 production in culture supernatant was examined by ELISA assay.The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of [3H]-TdR.RESULTS:Compared with control group,cultured DCs in the presence of P4 displayed less dendritic pseudopod,expressed low levels of MHC-II,CD40,CD80 and CD86,and exhibited weakly activity in stimulating the proliferation of allogeneic T cells.Increase in IL-10 production and decrease in IL-12 production were observed.CONCLUSION:P4 exerts negative effect on the maturation and immunologic function in dendritic cells from human peripheral blood.
