Adenovirus latent infection enhances the oxidant/antioxidant imbalance in rat alveolar epithelial cells
- VernacularTitle:腺病毒潜伏感染对大鼠肺泡上皮细胞氧化/抗氧化平衡的影响
- Author:
Yi FANG
;
Bing LI
;
Juan CHEN
;
Qicai LIU
;
Pixin RAN
- Publication Type:Journal Article
- Keywords:
Adenovirus latent infection;
E1A;
Rats;
Alveolar epithelial cells;
Lung diseases,obstructive
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the influence of adenovirus latent infection on the oxidant/antioxidant balance in rat alveolar epithelial cells.METHODS:The rat alveolar epithelial cells were stably transfected with the plasmid pE1Aneo and control plasmid pneo.GSH and MDA contents,the activities of major anti-oxidative enzymes including SOD,CAT,GPx,GST and ?-GCS were detected in oxidant stress.RESULTS:Adenovirus E1A expression repressed the activity of ?-GCS,and decreased GSH contents in oxidant stress.As a result,the activity of GPx and GST was decreased.The contents of MDA maintained high in oxidant stress.CONCLUSION:Adenovirus latent infection amplifies the oxidant/antioxidant imbalance in rat alveolar epithelial cells in oxidants stress,and adenovirus E1A protein decreases the activity of ?-GCS,which plays an important role in this process.
