The effect of diabetes mellitus on intimal hyperplasia in autogenous vein graft of rats
- VernacularTitle:糖尿病对大鼠自体移植静脉内膜增生的影响
- Author:
Xin WANG
;
Qiang ZHANG
;
Chengzuo ZHANG
;
Hongwei ZHANG
;
Xin HUO
- Publication Type:Journal Article
- Keywords:
Tunica Intima;
Hyperplasia;
Advanced Glycation end Products;
Graft Occlusion,Vascular
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of AGE-RAGE system on intimal hyperplasia in autogenous vein graft of diabetic rats.Methods The experiments were performed on 40 male Sprague-Dawley rats which divided into two groups:diabetes group and control group.Autogenous vein graft model was established in all rats.Specimens were harvested 7 days and 14 days after surgery for histological examination,and RAGE,NF-?B p65 and VCAM-1mRNA were measured by semi-quantitative reverse transcription-PCR.Western Blotting and immunohistochemistry were used to detect the protein expression of RAGE,NF-?B p65 and VCAM-1.At the same time,the levels of serum AGE were tested.Results Compared with non-diabetic rats of control group,the intimal hyperplasia of vein graft in diabetic rats obviously increased(P
