Dynamic effect of endothelin-converting enzyme on intimal hyperplasia after venous autograft
- VernacularTitle:内源性内皮素转化酶在自体移植静脉术后的动态变化
- Author:
Xicai LIU
;
Qingbin SONG
;
Cangang ZHANG
- Publication Type:Journal Article
- Keywords:
VeinsTransplantation,Autologous;
Tunica Intima,Hyperplasia;
Smooth Muscle Cell;
Endothelin-1;
Endothelin Converting Enzyme;
Immunohistochemistry;
RT-PCR
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effet of ET-1 and ECE on endothelial proliferation of autografted vein.Methods An animal model of the autogenous vein graft was established in 80 Wistar rats.The expression of ECE and EF-1 gene was determined by RT-PCR and immunohistological method to test the mRNA and protein expresion level respectively.Results PCNA positive smooth muscle cells appeared 6 hours after transplantation,increased with time,and reached a peak at 1 to 2 weeks.After 2 weeks,PCNA positive SMC in the tunica media began to decline and recovered to the 6 hour level at 8 weeks after the operation.mRNA of ECE increased with the time after the operation,reached a peak after 1-2 weeks,declined afterwards,and became stable at around 8 weeks.Expression change of ET1 was similar to the change of ECE,reached the peak after 1-2 weeks and was stable after 8 weeks(r=0.975).Conclusions The time and pattern of change of the pathologic process of intimal hyperplasia are in agreement with the expression of ET-1 and ECE,and suggests that ECE is involved in the process of intimal hyperplasia of vein graft.
