The feature of TCR-zeta chain expression in patients with CML by real-time quantitative PCR
- VernacularTitle:慢性粒细胞白血病病人TCR?链表达特点
- Author:
Si CHEN
;
Yangqiu LI
;
Shaohua CHEN
;
Lijian YANG
;
Xiuli WU
;
Dongzhi CEN
- Publication Type:Journal Article
- Keywords:
T-cell receptor ? chain;
Real-time quantitative PCR;
Leukemia,myeloid,chronic
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a real-time PCR technique for detection and quantification of TCR ? chain expression and to investigate TCR ? chain expression level in patients with chronic myeloid leukemia(CML).METHODS:Real-time PCR with SYBR GreenⅠ technology was used for detecting TCR ? chain expression level in peripheral blood mononuclear cells from 30 patients with CML and 30 normal individuals.?2-microglobulin gene(?2M) was used as an endogenous reference.Relative changes in TCR ? chain expression level were used by the 2-Ct method between patients with CML and normal individuals.RESULTS:The SYBR GreenⅠ real-time technique for quantitative detection of TCR ? chain expression levels was established successfully.The expression level of TCR ? chain in 18 patients with CML was reduced.However,the TCR ? chain expressed increased in 12 patients with CML.CONCLUSION:The TCR ? chain expression level is divided into down expression(60%) and over expression(40%) groups,and the down expression of TCR ? chain might related to cellular immunodeficiency in most of CML patients.
