Effect of amino-functional Fe_3O_4 nanoparticles-mediated pcDNA3.1(+)-p53 on HepG2 Cells in vitro
- VernacularTitle:磁性纳米介导的pcDNA3.1(+)-p53对HepG2细胞的作用
- Author:
Dequn OUYANG
;
Yangde ZHANG
;
Jiantai HE
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocellular;
Gene,p53;
Amino-functional Fe_3O_4 Nanoparticle;
Gene Therapy
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05).(2) The transfection rate and velocity could be increased by addition of extra-magnetic field.(3) Transfected-p53 obviously inhibited the growth of HepG2 at G1 phase;the growth inhibition rate of HepG2 was 46%.Conclusions(1) FFN is a good vector for gene transfection,it can successfully transfect the gene into target cells.(2) Transefected-p53 can effectively inhibit the growth of HepG2.This result can establish a foundation for animal experimental research of gene therapy of malignancy.
