Experiment study of fibrin gels as the substrate for cultivating human amniotic epithelial sheets in vitro
- VernacularTitle:纤维蛋白胶为载体构建人羊膜上皮细胞植片的实验研究
- Author:
Fang ZHAO
;
Jian CHEN
;
Jing WU
;
Jingtang XU
;
Qing ZHOU
;
Yanping WANG
;
Ling JIN
;
Songbin ZHAO
- Publication Type:Journal Article
- Keywords:
Amniotic epithelial cells,human;
Fibrin tissue adhesive;
Cell culture
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the feasibility of using human amniotic epithelial cells(HAECs) sheets,which are cultured by using a biodegradable fibrin sealant to re-establish corneal surface layer.METHODS:Human amniotic membrane(AM) harvested at the time of elective caesarean section was digested by collagenase and trypsin respectively to obtain HAECs.The HAECs were planted on Millicell culture dishes coated with biodegradable fibrin glue,and cultured by using the air-lifting cultivation.The HAECs sheets were investigated morphologically by Hematoxylin-Eosin staining,scanning electron microscopy(SEM) and were identified by cytokeratin immunohistochemistry.RESULTS:HAECs sheets were transparent and successfully cultured by using a biodegradable fibrin sealant.Most of the cultured cells were round or polygon and typical slabstone-like appearance.Many microvilli were observed on cell surfaces by SEM.Cytokeratin staining was positive.HAECs had stratificated growth tendency and became corneal-like stratificating epithelial cells.CONCLUSION:A commercially available fibrin sealant was an effective means of tissue engineering to create a carrier-free,transplantable HAECs sheets.The sheets were potential graft to re-establish corneal surface layer.
