Effect of Celecoxib on the Expression of Cyclooxygenase-2 in Atherosclerotic Tissues in Rabbits
- VernacularTitle:塞来昔布对兔动脉粥样硬化组织中环氧合酶-2的作用研究
- Author:
Lihua LI
;
Han LEI
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Cyclooxygenase-2;
Celecoxib;
Rabbit
- From:
China Pharmacy
2005;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observe the expression of cyclooxygenase-2(COX-2) in atherosclerotic tissue in rabbits and to discuss the association between COX-2 and atherosclerosis (AS) and evaluate the effect of cyclooxygenase-2(COX-2) inhibitor celecoxib (cele) on atherosclerosis progression in atherosclerotic model rabbits. METHODS: Eighteen New Zealand rabbits were randomly divided into three groups: normal control group, high cholesterol (HC) group, and celecoxib(20 mg?kg?d-1) group. After feeding for 14 weeks, the aortic atherosclerotic lesions and the ratio of aortic intima to media in rabbits were measured. RT-PCR, immunohistochemistry and Western Blot were used for the detection of expression of COX-2 mRNA and protein. RESULTS: In celecoxib-treated group compared with HC group, the atherosclerotic lesion area, the ratio of aortic intima to media, the expression of COX-2 mRNA and protein were all significantly reduced (P
