Determination of Icariin in Yangqiang Baoshen Pills by Column Chromatography-HPLC
- VernacularTitle:柱层析-HPLC法测定阳强保肾丸中淫羊藿苷的含量
- Author:
Xiuyun WANG
- Publication Type:Journal Article
- Keywords:
Yangqiang baoshen pill;
Column chromatography -HPLC;
Icariin;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To determine the content of Icariin in Yangqiang baoshen pills by column chromatography-HPLC. METHODS: The chromatographic column was octadecylsilane chemically bonded silica(200 mm?4.6 mm, 10 ?m) with mobile phase consisted of 0.075 mol?L-1 phosphoric acid -B cyanide (73∶27) at a detection wavelength of 270 nm. RESULTS: The linear range of Icariin was 10-18 ?g?mL-1(r=0.999 5) and the average recovery was 94.35%(RSD=1.12%,n=6).CONCLUSION: This method is simple, accurate, reproducibility and applicable for the quality control of Yangqiang baoshen pill.
