Proliferation Inhibition and Apoptosis of Human Gastric Cancer Cells Induced by Oridnin
- VernacularTitle:冬凌草甲素抑制人胃腺癌细胞生长和诱导凋亡作用研究
- Author:
Qingfeng XIN
;
Junhui CHEN
- Publication Type:Journal Article
- Keywords:
Oridonin;
Gastric carcinoma;
Apoptosis;
Mitochondria;
Endoplasmic reticulum;
Cell cycle
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the growth inhibition and apoptosis of BGC-823 gastric cancer cells induced by Oridonin and its mechanism. METHODS: The Oridonin-treated BGC-823 cells were detected using MTT, transmission electron microscopy (TEM), TUNEL and flow cytometry (FCM) etc. RESULTS: Oridonin(16, 32, and 64 ?g?mL-1) significantly inhibited the proliferation of BGC-823 cells in dose and time dependent manner, with the inhibition rates at 68.5%, 90.8%, and 94.7%, respectively within 24, 48, and 72 h after treatment with 64 ?g?mL-1 Oridnin. When treated with 32 ?g?mL-1 Oridonin for 2 h,mitochondria and endoplasmic reticulum were proliferated and swelled under TEM; At 8 h,vacuolization of mitochondria and endoplasmic reticulum were noted, the internal structure disappeared, and the nuclear chromatin margination vacuolization were in typical apoptosis change; At 24 h, BGC-823 cells were arrested at G2/M phase, and the apoptosis rate was 37.8% under FCM. CONCLUSION: Oridonin inhibiting proliferation and apoptosis of BGC-823 cells maybe related to G2/M phase arresting, and Oridonin induce apoptosis of BGC-823 cells may through mitochondria or endoplasmic reticulum apoptosis pathway.
